Biolog Phenotype MicroArray
     Serious Technology for Serious Microbiology January 2015
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Useful Links

Phenotype MicroArrays Used to Study Microalgae, Oomycetes, and Morphologically Distinct Bacteria

Phenotype MicroArray
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This issue focuses on microorganisms that phylogenetically distinct including photosynthetic microalgae, fungus-like eukaryotes, and bacteria with internal membrane compartments.  The number of species tested with Phenotype MicroArrays continues to expand and reach further across the phylogentic tree.  In fact, Phenotype MicroArrays can now be applied to mammalian cells.
 
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  Microalgal Metabolic Network Model Refinement Through High-Throughput Functional Metabolic Profiling
PM technology was used for systematic functional metabolic profiling of Chlamydomonas reinhardtii.  PM test results were used to experimentally verify metabolic model predictions and identify new substrate utilizations.  The study resulted in an expanded and optimized metabolic model, which serves as a foundation for the study of mutants and novel isolates.
 
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  Utilization of Carbon Sources by Pythium, Phytophthora, and Fusarium Species as Determined by Biolog MicroPlate Assay
This paper describes the carbon source utilization of two Oomycetes, which are fungus like, but in a distinct phylogenetic lineage. Two Phytophthora, one Pythium, and two Fusarium (Fungi) were tested in Biolog's GN2 carbon source utilzation microplate.  These data allowed these root pathogens from closed hydroponic systems to be divided into two major groups.
 
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  Characterization of a Planctomycetal Organelle: a Novel Bacterial Microcompartment for the Aerobic Degradation of Plant Saccharides
The authors investigated a conserved bacterial microcompartment (BMC) gene cluster that is apparently exlusive to Verrucomicrobia and Planctomycetes.  A knockout deficient in an aldehyde dehydrogenase gene showed reduced usage of a number of carbon sources including mannose containing substrates, and no usage of L-fucose.  Independent experiments confirmed that BMC's are required for growth on L-fucose and L-rhamnose.
 
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Phenotype MicroArray Technology
Biolog's Phentoype MicroArray technology enables researchers to evaluate nearly 2000 phenotypes of a microbial cell in a single experiment. This integrated system of cellular assays, instrumentation and bioinformatics software provides cellular knowledge that complements molecular information, helping you interpret and find the relevant aspects in massive amounts of gene expression or proteomics data. Through comprehensive and precise quantitation of phenotypes, researchers are able to obtain an unbiased perspective of the effect on cells of genetic differences, environmental change, exposure to chemicals or drugs, and more.
 
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