Biolog Phenotype MicroArray
     Serious Technology for Serious Microbiology February 2014
""
     
 
""

Useful Links

Virulence Studied with Phenotype MicroArrays

Phenotype MicroArray
""
"" "" ""
 
 
New publications illustrate the application of Phenotype MicroArray (PM) technology in virulence research. In this newsletter, we present three papers from the primary literature illustrating successful use of PM technology to further understand mechanisms of virulence and host cell-pathogen interaction.
 
"" "" ""
  Central Metabolism Controls Transcription of a Virulence Gene Regulator in Vibrio cholerae
Mutations in TCA cycle enzymes and the respiration linked sodium pump NADH:ubiquinone oxidoreductase (NQR) increased transcription of toxT. Phenotype MicroArrays were used to demonstrate a TCA cycle defect in the NQR mutant. Since NQR activity is inhibited in TCA enzyme mutants, they propose that NQR regulates toxT transcription via the TCA cycle. These and other data led to the proposal that toxT transcription is regulated by acetyl-coA or a close derivative.
 
""""""
  Absenceof Membrane Phosphatidylcholine Does Not Affect Virulence and Stress Tolerance Phenotypes in the Opportunistic Pathogen Pseudomonas aeruginosa
Based on genomic observations, the authors hypothesized a role for phosphatidyl choline (PC) in virulence or survival.  A pcs mutation was created in strain PAO1.  In an exhaustive effort to find a phenotype, Phenotype MicroArrays were employed.  No reproducible differences were found.  It is speculated that perhaps PC may play a role in localizing specific membrane proteins.
 
""""""
  PoxA, YjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica
An interaction between lysyl tRNA-synthetase and 2,3-β-lysine aminomutase was found to be critical for virulence.  Phenotype MicroArray profiles of PoxA and YjeK mutants were a phenocopy of each other.  This and other data allowed the authors to propose that PoxA and YjeK act in a common pathway to modify elongation factor P, affecting the expression of a subset of virulence proteins.
 
""""""
 
Phenotype MicroArray Technology
Biolog's Phentoype MicroArray technology enables researchers to evaluate nearly 2000 phenotypes of a microbial cell in a single experiment. This integrated system of cellular assays, instrumentation and bioinformatics software provides cellular knowledge that complements molecular information, helping you interpret and find the relevant aspects in massive amounts of gene expression or proteomics data. Through comprehensive and precise quantitation of phenotypes, researchers are able to obtain an unbiased perspective of the effect on cells of genetic differences, environmental change, exposure to chemicals or drugs, and more.
 
""""""
""""""