Biolog Phenotype MicroArray
     Serious Technology for Serious Microbiology June 22, 2010
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Phenotype MicroArrays Used in Food Safety Research
New publications illustrate the application of Phenotype MicroArray (PM) technology in food safety and food protection research. In this newsletter, we present three papers from the primary literature illustrating successful use of PM technology further understand the biology of food pathogens.
 
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 Genes involved in yellow pigmentation of Cronobacter sakazakii ES5 and influence of pigmentation on persistence and growth under environmental stress.

Transposon induced colorless mutants were compared to the colored WT strain and analyzed in multiple ways including Phenotype MicroArrays. Colorless mutants grew at higher rates under osmotic stress. Other findings include relative sensitivity to UVB and dessication. These analyses of growth and persistance enhance our understanding of Cronobacter physiology and the role of pigmentation.

The abstract can be viewed here:

 
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 Differential carbon source utilization by Campylobacter jejuni 11168 in response to growth temperature variation.

These authors used PM1 and PM2 to create a carbon source utilization profile at human body temperature (37C) and chicken body temperature (42C). Amino acids and organic acids were used as sole carbon sources. These data will aid media development for detection, isolation, and study of Campylobacter.

The abstract can be viewed here:

 
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 Selection and Characterization of Cellulose-Deficient Derivates of Shiga Toxin-Producing Escherichia coli.

Pairs of cellulose-deficient and cellulose-proficient strains were created to address the role of cellulose in protecting Shiga-Toxin producing E. coli. Although cellulose deficiency did not alter the serological property or PFGE profile under ideal conditions, PM testing revealed growth differences in several adverse environmental conditions.

The abstract can be viewd here:

 
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Phenotype MicroArray Technology
Biolog's Phentoype MicroArray technology enables researchers to evaluate nearly 2000 phenotypes of a microbial cell in a single experiment. This integrated system of cellular assays, instrumentation and bioinformatics software provides cellular knowledge that complements molecular information, helping you interpret and find the relevant aspects in massive amounts of gene expression or proteomics data. Through comprehensive and precise quantitation of phenotypes, researchers are able to obtain an unbiased perspective of the effect on cells of genetic differences, environmental change, exposure to chemicals or drugs, and more.
 
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Applications for Phenotype MicroArrays
Phenotype MicroArrays add a fresh cell-based perspective to a wide range of activities.

  • Discovery research
  • Cell line characterization
  • Mutant and mutation characterization
  • Determination of gene function
  • Culture medium optimization
  • BioProcess development and improvement
  • BioProcess QC and validation
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