Biolog Phenotype MicroArray
     Serious Technology for Serious Microbiology July 2012
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  • Fungal and Yeast Biology Studied with Phenotype MicroArrays


    Phenotype MicroArray
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    One review article and two papers illustrate the use of Phenotype MicroArray technology in the study of fungal biology. Biolog offers PM panels for both metabolic profiling and chemical sensitivity testing of yeast and fungi.
     
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    A Novel Fungal Family of Oligopeptide Transporters Identified by Functional Metatranscriptomics of Soil Eukaryotes
    Marmeisse and colleagues converted polyadenylated soil RNA to cDNA for expression in a yeast mutant unable to transport peptides. They recovered transformants containing environmentally sourced peptide transport genes that functionally complemented the yeast mutant. Phenotype MicroArrays were used to functionally characterize the transporters and the genes were also mapped phylogenomically. Using a Xenopus oocyte model, one of the transporter genes was shown to induce peptide-dependent ion currents.

     
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    Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
    Comparative genomics showed that the Onygenales family, including Paracoccidioides, have lost genes in carbohydrate metabolism. PM analysis of a non-pathogenic species of Onygenales showed a restricted range of carbon sources used including plant sugars and cell wall components. Additionally, use of gelatin, amino acids, and peptides, suggest that this dimorphic family of fungi can live a dual lifestyle using plant substrates in the soil and animal protein in the host.

     
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    Global Nutrient Profiling by Phenotype MicroArrays: a Tool Complementing Genomic and Proteomic Studies in Conidial Fungi
    Atansova and Druzhina reviewed the use of Phenotype MicroArrays in nutrient profiling of conidial fungi. They emphasized optimization of testing protocols, measurement of cell mass, reproducibility of the technology, and the importance of kinetic data. They also reviewed inter and intra-specific variability, profiling of mutant strains, and other applications.

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    Phenotype MicroArray Technology
    Biolog's Phentoype MicroArray technology enables researchers to evaluate nearly 2000 phenotypes of a microbial cell in a single experiment. This integrated system of cellular assays, instrumentation and bioinformatics software provides cellular knowledge that complements molecular information, helping you interpret and find the relevant aspects in massive amounts of gene expression or proteomics data. Through comprehensive and precise quantitation of phenotypes, researchers are able to obtain an unbiased perspective of the effect on cells of genetic differences, environmental change, exposure to chemicals or drugs, and more.
     
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    Applications for Phenotype MicroArrays
    Phenotype MicroArrays add a fresh cell-based perspective to a wide range of activities.

  • Discovery research
  • Cell characterization
  • Mutant and mutation characterization
  • Determination of gene function
  • Transporter substrate assignment
  • Chemical phenomics
  • Strain discrimination
  • Genome annotation
  • Metabolic modeling
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