Biolog Phenotype MicroArray
     Serious Technology for Serious Microbiology September 2013
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Useful Links

Reporter Genes and Secondary Metabolites

Phenotype MicroArray
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Biolog PM plates can be used with your reporter gene or assay of secondary metabolite.  Think of the plates as a source of diverse culture conditions that you can use to grow your cells and use the resulting culture to measure DNA, RNA, protein, lipids, or other metabolites.  The first paper uses a novel assay for toxin production (Mammalian Biolog PM Tests).  The second paper uses GFP (a fluorescent reporter).  The third paper uses LCMS to detect secondary metabolites.
 
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  Using Phenotype MicroArrays to Determine Culture Conditions That Induce or Repress Toxin Production by Clostridium difficile and Other Microorganisms
Lei and Bochner developed a new assay for toxin quantification.  They combined the new assay with Phenotype MicroArrays as a source of diverse culture conditions.  After testing 768 conditions, they identified metabolic substrates that either induce or repress toxin production.  They propose that this new functional cytotoxin quantification method be applied to toxigenic microorganisms.
 
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 Nutrient Profiling Reveals Potent Inducers of Trichothecene Biosynthesis in Fusarium graminearum
 Gardiner et al., used a GFP reporter of the TRI5 gene to identify metabolic substrates that induce the tricothecene biosynthetic pathway in Fusarium graminearum.  Specific carbon sources induced GFP expression and tricothecene production.  Based on these results, they propose that the products of the arginine-polyamine pathway may play a role in tricothecene biosynthesis.
 
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 Application of Biolog FF MicroPlate for Substrate Utilization and Metabolite Profiling of Closely Related Fungi
The FF microplate was used as a diverse set of culture conditions.  Resulting cultures were assayed for substrate utilization, growth, and secondary metabolites (measured by LCMS).  The resulting fingerprints assisted in optimization of culture conditions for secondary metabolites.
 
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Phenotype MicroArray Technology
Biolog's Phentoype MicroArray technology enables researchers to evaluate nearly 2000 phenotypes of a microbial cell in a single experiment. This integrated system of cellular assays, instrumentation and bioinformatics software provides cellular knowledge that complements molecular information, helping you interpret and find the relevant aspects in massive amounts of gene expression or proteomics data. Through comprehensive and precise quantitation of phenotypes, researchers are able to obtain an unbiased perspective of the effect on cells of genetic differences, environmental change, exposure to chemicals or drugs, and more.
 
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