Biolog Phenotype MicroArray
     Serious Technology for Serious Microbiology November 2011
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Cystic Fibrosis (CF) Microbial Pathogenesis Studied with Phenotype MicroArrays

Phenotype MicroArray
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Three recent articles from the primary literature illustrate the utility of Phenotype MicroArray technology in the study of Cystic Fibrosis lung pathogens. Topics include analysis of changes over time during lung infection, comparative genomics of Pseudomonads, and mechanisms of antibiotic resistance.
 
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  Evolutionary Dynamics of Bacteria in a Human Host Environment
Using patient isolates spanning 200,000 generations and three experimental techniques, Molin and colleagues investigated adaptation of the DK2 lineage to the host airway. Computational analysis of both transcriptome and Phenotype MicroArray data showed a group of strains isolated in 1979-2008 are more similar to each other than to the strains isolated in 1973. The DK2 lineage underwent a period of rapid change followed by a long period of little change.

The abstract can be downloaded here:
- Evolutionary Dynamics of Bacteria in a Human Host Environment

 
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 Reconciliation of Genome-Scale Metabolic Reconstructions for Comparative Systems Analysis
In order to perform metabolic network comparisons between organisms, Martin dos Santos, Papin and colleagues developed a new process called metabolic network reconciliation. Biolog phenotyping was used to validate the reconstruction. The validated reconstruction was used for comparison of metabolic flexibility in an effort to understand the underlying Pseudomonad biology. This group previously used a metabolic reconstruction of Pseudomonas aeruginosa tailored to the metabolic states of lineages isolated from lungs at different time points over a relatively short timecourse.

The abstracts can be viewed here:
- Reconciliation of Genome-Scale Metabolic Reconstructions for Comparative Systems Analysis

- Metabolic Network Analysis of Pseudomonas aeruginosa during Chronic Cystic Fibrosis Lung Infection

 
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 Deciphering the Role of RND Efflux Transporters in Burkholderia cenocepacia
Burkholderia cenocepacia is another CF lung pathogen that exhibits relative antibiotic resistance. Buroni and colleagues studied RND (Resistance-Nodulation-Cell Division) efflux pumps to understand their role in the efflux of toxic compounds. Two of 16 RND efflux pumps were deleted and analyzed in several ways to understand their role in compound efflux. PM Sensitivity panel data and other data supported a significant role for RND-9, but not for
RND-4.

The abstract can be downloaded here:
- Deciphering the Role of RND Efflux Transporters in Burkholderia cenocepacia

 
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Phenotype MicroArray Technology
Biolog's Phentoype MicroArray technology enables researchers to evaluate nearly 2000 phenotypes of a microbial cell in a single experiment. This integrated system of cellular assays, instrumentation and bioinformatics software provides cellular knowledge that complements molecular information, helping you interpret and find the relevant aspects in massive amounts of gene expression or proteomics data. Through comprehensive and precise quantitation of phenotypes, researchers are able to obtain an unbiased perspective of the effect on cells of genetic differences, environmental change, exposure to chemicals or drugs, and more.
 
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