ÿþ<!-- saved from url=(0022)http://internet.e-mail --> <style type="text/css"> <!-- .style1 {font-size: 18px} .style5 {color: #003399; font-family: Arial, Helvetica, sans-serif; font-size: 2; } .style7 {font-size: small} .style8 {font-family: Arial, Helvetica, sans-serif; font-size: small; } .style9 {color: #003399} --> </style> <!-- startprint --> <div align="left"> <table width="728" border="0" cellspacing="0" cellpadding="0"> <!--DWLayoutTable--> <tr> <td width="730" height="38" valign="top"><img src="http://www.biolog.com/images/NewsletterHeader.jpg" width="730" height="98"></td> </tr> <tr> <td height="19" align="left" valign="middle" bgcolor="#FFFF99"><img src="http://www.biolog.com/images/menubar.gif" width="621" height="20" border="0" usemap="#MenuBar"><img src="http://www.biolog.com/images/V10_I3.gif" width="108" height="23"></td> </tr> </table> <table width="728" border="0" cellpadding="0" cellspacing="0"> <!--DWLayoutTable--> <tr> <td width="166" height="1958" valign="top"><table width="166" border="0" cellspacing="0" cellpadding="0"> <!--DWLayoutTable--> <tr> <td colspan="3" height="30" valign="top"><img src="http://www.biolog.com/images/but_123_over.gif" width="166" height="29"></td> </tr> <tr> <td valign="middle" height="18" align="center" colspan="3"><img src="http://www.biolog.com/images/lineH100.gif" width="100" height="7"></td> </tr> <tr> <td height="30" width="28"></td> <td colspan="2" valign="top"> <div align="left"> <a href="http://www.biolog.com/pmTechDesOver.html" class="style11"><font size="2" face="Arial" class="style11">PM Technology<br> Description/Overview</font></a></div> </td> </tr> <tr valign="middle" align="center"> <td colspan="3" height="18"><img src="http://www.biolog.com/images/lineH100.gif" width="100" height="7"></td> </tr> <tr> <td height="30"></td> <td colspan="2" valign="top"><a href="http://www.biolog.com/drugDisc.html" class="style11"><font size="2" face="Arial" class="style11">Drug Discovery<br> Using PMs</font></a></td> </tr> <tr valign="middle" align="center"> <td height="18" colspan="3"><img src="http://www.biolog.com/images/lineH100.gif" width="100" height="7"></td> </tr> <tr> <td height="30"></td> <td colspan="2" valign="top"><a href="http://www.biolog.com/pmMicrobialCells.html" class="style11"><font size="2" face="Arial" class="style11">PMs for<br> Microbial Cells</font></a></td> </tr> <tr valign="middle" align="center"> <td height="18" colspan="3"><img src="http://www.biolog.com/images/lineH100.gif" width="120" height="7"></td> </tr> <tr> <td height="30"></td> <td colspan="2" valign="top"><a href="http://www.biolog.com/pmMammalianCells.html" class="style11"><font size="2" face="Arial" class="style11">PMs for<br> Mammalian Cells</font></a></td> </tr> <tr valign="middle" align="center"> <td height="18" colspan="3"><img src="http://www.biolog.com/images/lineH100.gif" width="100" height="7"></td> </tr> <tr> <td height="30"></td> <td colspan="2" valign="top"><a href="http://www.biolog.com/mID_section_13.html" class="style11"><font size="2" face="Arial, Helvetica, sans-serif" class="style11">Publications on<br> PM Technology</font></a></td> </tr> <tr align="center" valign="middle"> <td height="18" colspan="3"><img src="http://www.biolog.com/images/lineH100.gif" width="100" height="7"></td> </tr> <tr> <td height="30"></td> <td colspan="2" valign="top"><a href="http://www.biolog.com/PM_Services.html" class="style11"><font size="2" face="Arial" class="style11">Phenotype MicroArray<br> Services</font></a></td> </tr> <tr valign="middle" align="center"> <td height="18" colspan="3"><img src="http://www.biolog.com/images/lineH100.gif" width="100" height="7"></td> </tr> <tr> <td height="30"></td> <td colspan="2" valign="top"><a href="http://www.biolog.com/accesstoPMTech.html" class="style11"><font size="2" face="Arial" class="style11">Access to PM<br> Technology</font></a></td> </tr> <tr valign="middle" align="center"> <td height="18" colspan="3"><img src="http://www.biolog.com/images/lineH100.gif" width="100" height="7"></td> </tr> <tr> <td height="34" colspan="3" valign="top"><a href="http://biolog.com/microID.html"><img name="Image10" border="0" src="http://www.biolog.com/images/but_microbialID.gif" width="166" height="34"></a></td> </tr> <tr> <td height="1385" colspan="2" valign="top"><img src="http://www.biolog.com/images/leftBlueBar.gif" width="32" height="100%" align="top"></td> <td width="134"> </td> </tr> <tr> <td height="140"></td> <td></td> <td></td> </tr> <tr> <td height="1"></td> <td width="4"></td> <td></td> </tr> </table></td> <td width="562" rowspan="2" valign="top"> <table width="98%" border="0" cellspacing="0" cellpadding="0"> <!--DWLayoutTable--> <tr> <td width="490" height="20"></td> <td width="4"></td> <td width="57"></td> <td width="5"></td> <td width="5"></td> </tr> <tr> <td rowspan="3" align="left" valign="top" height="310"><h1 align="center" class="style1 style1"><font face="Arial, Helvetica, sans-serif" color="#FF0000">Two new publications illustrate the use of PM Technology for validating bioinformatic predictions, identifying new pathways, and refining <I>in silico</I> models.</font></h1> </font></span> <li class=style5><font size="2"><a href="http://www.ncbi.nlm.nih.gov/pubmed/18833300?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum">High-Throughput Phenotypic Characterization of Pseudomonas aeruginosa Membrane Transport Genes.</a><font face="Arial,Helvetica, sans-serif" size"2" Color="#000000"> Johnson DA, Tetu SG, Phillippy K, Chen J, Ren Q, Paulsen IT. J Craig Venter Institute, Rockville, Maryland, United States of America. Paulsen and colleagues used PM metabolic assay to investigate the accuracy of bioinformatic predictions for carbon and nitrogen membrane transport genes. Twenty-seven of the 78 knockouts gave clear transporter phenotypes, and of these only 12 (44%) precisely matched the predicted annotation. In 10, a more precise annotation was obtained, and in 5 (18%), a significant re-annotation was enabled. New transporters were found for hydroxy-L-proline, N-acetyl-L-glutamate and histamine. The latter is the first histamine transporter to be identified. <h1> </font></h1> </font></span> </font></h1> <li class=style5><font size="2"><a href="http://www.ncbi.nlm.nih.gov/pubmed/18974823?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum">Genome-scale reconstruction and analysis of the Pseudomonas putida KT2440 metabolic network facilitates applications in biotechnology.</a><font face="Arial,Helvetica, sans-serif" size"2" Color="#000000"> PuchaBka J, Oberhardt MA, Godinho M, Bielecka A, Regenhardt D, Timmis KN, Papin JA, Martins dos Santos VA. Synthetic and Systems Biology Group, Helmholtz Center for Infection Research (HZI), Braunschweig, Germany. Biolog carbon utilization assays were used to validate an in silico reconstruction of P. putida core metabolism. <br><br> </a> <br> </font></span> </a<br> </ul></td> <td height="23"> </td> </tr> <tr> <td height="32">&nbsp;</td> <td colspan="2" align="center" valign="top"><div align="center"><strong><font face="Arial, Helvetica, sans-serif" size="2" color="#FF0000"><a href="http://www.biolog.com/pdf/V10_I3.pdf"><font color="#FF0000">Printable PDF</font></a></font></strong></div></td> <td>&nbsp;</td> </tr> <tr height="255"> </tr> <tr height="18"> <td height="18" colspan="5"><img src="http://www.biolog.com/images/lineH100.gif" width="550" height="7"></td> </tr> <tr> <td height="1534" colspan="3" valign="top"> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <!--DWLayoutTable--> <tr> <td width="551" height="1534" valign="top"> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <!--DWLayoutTable--> <tr> <td width="551" height="1534" valign="top" class="text"> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <!--DWLayoutTable--> <tr valign="top"> <td width="551" height="1534" valign="top"> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <!--DWLayoutTable--> <tr height="6"> <td width="1" height="6"></td> <td width="262" height="6"></td> <td width="14" height="6"></td> <td width="10" height="6"></td> <td width="32" height="6"></td> <td width="31" height="6"></td> <td width="184" height="6"></td> <td width="17" height="6"></td> </tr> <tr height="18"> <td height="18" colspan="8" valign="top" class="title"> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <!--DWLayoutTable--> <tr> <td width="542" height="18"></td> </tr> </table></td> </tr> <tr height="10"> <td height="10"></td> <td colspan="7" valign="top" class="subTitleBold" height="10"><a name="whatPMare"></a></td> </tr> <tr> <td height="229"></td> <td colspan="6" valign="top" class="text style4 style6"></a><font face="Arial, Helvetica, sans-serif" size="2" color="#003399"><u class="title style3"></li><u class="title style3"><font face="Arial, Helvetica, sans-serif" color="#FF0000"><b>Useful links</b></font></u></u></font> <div align="left"> <ul> <li class="style5"><a href="http://www.biolog.com/register.htm"><font face="Arial, Helvetica, sans-serif" color="#0000ff" size="2">Register with Biolog to receive regular updates on your area of interest</font></a>.</li> <li class="style5"><a href="http://www.biolog.com/pressRelease.html" target="_blank"><font face="Arial, Helvetica, sans-serif" color="#0000ff" size="2">New Biolog Press Releases</font></a>. </li> <LI class=style5><A class=style7 href="http://www.biolog.com/phenoMicro.html"><FONT face="Arial, Helvetica, sans-serif" color=#0000ff size=2>For more information on PM technology</FONT></A></LI> <LI class=style5><A class=style7 href="http://www.biolog.com/PM_Services.html"><FONT face="Arial, Helvetica, sans-serif" color=#0000ff size=2>For information on Biolog's Phenotype MicroArray Services</FONT></A></LI> <LI class=style5><SPAN class=style8><A href="http://www.biolog.com/mID_section_13.html"><FONT face="Arial, Helvetica, sans-serif" color=#0000ff size=2>To view Biolog's PM publications and bibliography</FONT></A></SPAN></LI> <LI class=style5><A class=style7 href="mailto:pmmarketing@biolog.com"><FONT face="Arial, Helvetica, sans-serif" color=#0000ff size=2>To request sales information</FONT></A></LI> <LI class=style5><font face="Arial, Helvetica, sans-serif" color="#003399" size="2">"Forward-to-a-friend" link can be found at the bottom of the page.</font></LI> </ul> </div> <p><font face="Arial, Helvetica, sans-serif" size="2" color="#003399"><u class="title style3"><u class="title style3"><font face="Arial, Helvetica, sans-serif" color="#FF0000"><b>Brief description of PM technology</b></font></u></u></font></p> </td> <td> </td> </tr> <tr> <td height="9"></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr> <tr> <td height="224"></td> <td colspan="2" align="left" valign="middle" class="text"><p align="justify" class="style5"><font face="Arial, Helvetica, sans-serif" size="2" color="#003399">Phenotype MicroArray&trade; technology tests cellular phenotypes. Included in the tests are assays of basic cellular nutritional pathways for C, N, P, and S metabolism, osmotic and pH and sensitivity, and sensitivity to chemical agents.<br></font><font face="Arial, Helvetica, sans-serif" size="2" color="#003399"><br> The most common application is to assess the phenotypic effects of mutations. A change in genotype of a cell should lead to one or more changes in phenotype. PMs allow testing of knock-out or knock-in mutants to help discern the biological changes that occur consequent to genetic changes.</font></p> </td> <td> </td> <td colspan="4" align="right" valign="middle" class="text"> <p align="right"> <img width="264" height="215" src="http://www.biolog.com/images/Newsletter_clip_image002_0000.jpg"> </p></td> </tr> <tr> <td height="160"></td> <td colspan="5" align="center" valign="middle" class="text"> <p align="left"> <img width="328" height="149" src="http://www.biolog.com/images/Newsletter_clip_image002_0002.jpg"> </p></td> <td colspan="2" valign="top" class="text"> <p align="justify"> <span class="style5"><font face="Arial, Helvetica, sans-serif" size="2" color="#003399">Another common application is phenotypic characterization of a collection of related strains. For example, it is possible to determine the phenotypic relatedness of a collection of isolates of a given species. PM analysis has been successfully implemented for a wide variety of model microbial cells including <em>Escherichia coli, </em></font></span></p></td> </tr> <tr> <td height="48"></td> <td colspan="7" valign="top" class="text"> <p align="justify" class="style5"><font face="Arial, Helvetica, sans-serif" size="2" color="#003399"> <em><font face="Arial, Helvetica, sans-serif" size="2" color="#003399"><em>Salmonella enterica, Pseudomonas</em> <em>aeruginosa, <font face="Arial, Helvetica, sans-serif" size="2" color="#003399"><em>Pseudomonas putida, <font face="Arial, Helvetica, sans-serif" size="2" color="#003399"><em>Sinorhizobium meliloti, </em></font></em></font></em></font>Saccharomyces cerevisiae, Bacillus subtilis, Bacillus cereus, Shewanella oneidensis, Proteus mirabilis</em>, and many other species. In the last year, Biolog has successfully developed universal protocols that allow us to test most species of interest. Contact us for details.<br> </font><font face="Arial, Helvetica, sans-serif" size="2" color="#003399"><br> </font></p> </td> </tr> <tr> <td height="48"></td> <td colspan="7" valign="top" class="text"> <p align="justify" class="style5"><font face="Arial, Helvetica, sans-serif" size="2" color="#003399">Phenotype MicroArray&trade; technology uses Biolog's OmniLog&reg; instrument, which is also used for microbial identification. The OmniLog&reg; automatically incubates, reads and interprets the Biolog Phenotype MicroArray MicroPlates&trade;. It continuously processes samples but allows </font></p></td> </tr> <tr> <td height="160"></td> <td colspan="4" valign="top" class="text"> <p align="justify" class="style5"><font face="Arial, Helvetica, sans-serif" size="2" color="#003399">the user complete access at any time during a sample run. Samples can be loaded when ready and removed when complete without disturbing other samples still in-process. Inside the Reader there are 25 trays. Each tray holds 2 MicroPlates, giving the Reader a total capacity to incubate and read 50 MicroPlates. Before the user inoculates the appropriate MicroPlates, they log the MicroPlate information into the OmniLog&reg; software. </font></p></td> <td colspan="3" align="center" valign="middle" class="text"> <p align="right"> <img width="210" height="130" src="http://www.biolog.com/images/Newsletter_clip_image002_0003.jpg"> </p></td> </tr> <tr> <td height="288"></td> <td align="center" valign="middle" class="text"> <p align="left"> <img width="245" height="271" src="http://www.biolog.com/images/Newsletter_clip_image002_0004.jpg"> </p></td> <td colspan="6" valign="top" class="text"> <p align="justify" class="style5"><font face="Arial, Helvetica, sans-serif" size="2" color="#003399">By simply following the software's instructions, the user then opens the door of the OmniLog&reg; Reader and places each MicroPlate in the appropriate tray slot indicated by the software. Once all the MicroPlates are loaded and the door of the Reader is closed, the OmniLog&reg; software takes over the responsibility for incubating, reading, saving, and printing the results. The OmniLog&reg; PM System utilizes Windows-based software with an elegantly simple user interface. The status of all the samples can be observed by simply looking at the Read menu screen of the OmniLog&reg; PM System software. All of the information for a specific MicroPlate is contained on a single line of this screen. Once the system has completed a specific MicroPlate it indicates this status with a check mark icon. </font><FONT face=Arial size=2><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">A clock icon is used for those samples that are still incubating</SPAN></FONT></p></td> </tr> <tr> <td height="80"></td> <td colspan="7" valign="top" class="text"> <p align="justify" class="style5"><font face="Arial, Helvetica, sans-serif" size="2" color="#003399"><font face="Arial, Helvetica, sans-serif" size="2" color="#003399">because a result has not yet been determined. The OmniLog</font>&reg;<font face="Arial, Helvetica, sans-serif" size="2" color="#003399"> PM System's software is extremely flexible and can be integrated into virtually any laboratory's workflow. </font>New MicroPlates can be entered into the Reader and MicroPlates that have already completed can be removed from the Reader at almost any time. This allows the user to either perform experiments one at a time or batch them.<br> <br> </font></p></td> </tr> <tr> <td height="70"></td> <td colspan="7" valign="top" class="text"> <p><font face="Arial, Helvetica, sans-serif" size="2" color="#003399">PM Technology and the OmniLog&reg; instrument are ideal for a core facility or for purchase under a shared equipment grant.</font></p> <ul> </ul></td> </tr> <tr> <td height="19"></td> <td colspan="7" align="center" valign="middle"><img src="http://www.biolog.com/images/lineH100.gif" width="550" height="7"> <div align="right"></div></td> </tr> <tr> <td height="108"></td> <td colspan="7" valign="top"><div align="justify"><font class="text style4"><br> <font face="Arial, Helvetica, sans-serif" size="2" color="#003399">All content copyright Biolog, Inc. &copy; 2005. All trademarks and registered trademarks are the property of their respective companies. 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