Advanced Phenotypic Analysis

 
 
 
 

Phenotype MicroArray Fully Automated Systems

  • All OmniLog PM-M Systems include the OmniLog incubator/reader, computer, OmniLog PM Software, OmniLog User Guide, Pipettor, Training and a one year warranty. Combo sytems can be used for MI identification as well as reading Phenotype MicroArrays
  • Combo Systems include OmniLog Phenotype MicroArray Mammalian (PM-M) Software

Phenotype MicroArray Mammalian Systems

Catalog Number Description
96161
OmniLog® PM-M System
The OmniLog PM-M system has the capacity to incubate and monitor up to 50 PM-M MicroPlates for automated cell based metabolic analysis.

Size: 21 in (53 cm) W | 32 in (81 cm) | 23 in (58 cm) d
Power: 100 to 240 volts, 50 to 60 HZ
Operating Temp Range: 18° to 28° C
Operating Humidity Range: 20% to 80% non-condensing
Incubation Temperature Range: 22° to 45° C
Temp Consistency: +-2° C in the tray chamber
Incubation Humidity Range: Ambient
Test Capacity: 50 microplates
Reading Cycle Time: Upto 25 trays with 50 microplates in 15 mins
Temperature Control: Input of set temperature by external computer
Temperature Indication: Output to external computer and 7 segment display (green)
Other Indicators: Power On (green), Interrupt On (red), and Not at Temperature (red)
Monitor: LCD flat panel, 17 in viewable image size

Technical Document Downloads

Safety & Install Instructions
OmniLog Temperature Stability

Related Articles

Visualization and Curve-Parameter Estimation Strategies for Efficient Exploration of Phenotype Microarray Kinetics
Assay of the Multiple Energy-Producing Pathways of Mammalian Cells

FAQ

  1. OmniLog provides important advantages over end-point reads on a microplate reader in kinetic reading and throughput.

    Although end-point reads (when read at the proper time) can give partial quantitative information, they are usually just one data point, a non-averaged value with high statistical variance.

    The best information from the PMM assays is obtained by measuring initial rates of energy production, based on multiple reads. Using Biolog's proprietary tetrazolium dyes, the kinetics of dye reduction and purple color formation are initially linear with time. The OmniLog and OmniLog Software allow you to truncate the assays to their linear phase and accurately determine these initial metabolic rates for each well of each plate. Furthermore, dye reduction rates are independent of initial well color density, which may vary. Using OmniLog, no preliminary experiments need to be performed to determine the optimal time window for data collection because the kinetic record contains the entire time course. The kinetic read capability enables the relative rates of energy flux generation (or alterations) to be assigned to each substrate or metabolic effector.

    Throughput is a second major advantage. OmniLog has a capacity to assay 16 plates at 5 minute read intervals, or 50 plates at 15 minute read intervals.The large 50 plate capacity of the OmniLog enables multiple experiments (incorporating different media formulations, cell types, or chemical agents) to be run simultaneously.

  2. The software allows differences in substrate metabolism and rates of metabolism to be quickly assessed and displayed. Each pre-loaded substrate has a KEGG database identifier number. The software allows substrates of interest to be quickly matched to their respective pathway in KEGG. Data collected over many months of experiments are stored in standard formats and can be readily retrieved and compared.
 
 

     


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