Phenotype MicroArrays™ for Mammalian Cells

Phenotype MicroArray Mammalian assays are cell-based assays used to investigate up to 1,400 metabolic and chemical sensitivity phenotypes.

They use 96-well plates pre-loaded with carbon and nitrogen substrates, ions, stimulatory and inhibitory compounds such as hormones/cytokines and anti-cancer agents. Cells of interest are added to the plates along with Biolog’s proprietary redox dye. The generation of energy-rich NADH by the cells reduces the redox dye and brings about a color change which can be read and analyzed by Odin.

Applications

Phenotype MicroArray Mammalian Assays can be used for research in a diverse range of applications, including:

  • Correlating phenotypes with genotypes
  • Cell line and bioprocess improvement
  • Cellular metabolism, metabolic disorders, nutrition
  • Cell energetics, growth and death
  • Cell line QC and authentication
  • Drug discovery research and toxicity screening
  • Studying metabolic reprogramming in cancer and anti-cancer drug sensitivity

There are 14 panels designed to interrogate metabolic pathways along with ionic, osmotic and pH effects, and 10 panels to assess the sensitivity to various antimicrobials with different mechanisms of action.

Plate NameDescription
PM-M 1-4 MicroplatesMammalian metabolite utilization assays
PM-M 5 MicroplateMammalian cation/anion sensitivity assays
PM-M 6-8 MicroplatesMammalian hormone/metabolic effector assays
PM-M 11-14 MicroplateMammalian chemosensitivity assays
Checking for Growth with odin

See what’s happening in two ways.

Odin measures a reporter dye over time at one wavelength to measure NADH production, effectively reporting the rate of metabolic respiration.

It also measures optical density (OD) at a second wavelength to determine how quickly the microbes are dividing. Taken together, you get a full picture of how your microbes grow best over time.

If you’re focused on cell growth, just leave the dye out. Odin automatically compares cell growth and metabolic activity curves to provide you with a clearer picture of how fast or slow your microbes are growing.

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